Team Rotavirus
Team leader : Didier Poncet
Team leader : Didier Poncet
The rotavirus is the leading cause of gastroenteritis in young mammals, and is responsible for hundred of thousand deaths every year in the world. The viral particle is constituted by three concentric protein coats, surrounding a genome made of 11 segments of double stranded RNA . Several steps of the viral cycle remain still badly understood as, for instance, the mechanism of entry of the virus and the making of viroplasm (which are intracellular concentration of viral proteins where viral replication takes place).
A reverse genetic system should allow to study the biological mechanisms leading to the encapsidation in each viral particles, of all the eleven genes. The group investigate some of the stages of the viral cycle which could constitute targets for anti viral drugs. The team studies more particularly interactions between viral proteins and RNA and between viral proteins and cellular proteins. Through several collaboration, the team is also involved in the study of new strategies of vaccination against the rotavirus by the use of viral-like particles (VLPs).
- control of the translation of viral and cell mRNAs
- selection and of encapsidation of RNA viral
- mechanisms of virus entry and of viroplasms
- use of viral-like particles for vaccination against rotavirus
- Researchers
-
- Mariela Duarte, MdC Evry
- Didier Poncet - Team Leader, DR2 INRA
- Technical Staff
-
- Annie Charpilienne, IE1 INRA
- Patrice Vende, IE2 INRA
- Cécile Laroche, ATP INRA
- Non-Permanent Staff
-
- Matthieu Saguy, Post-doctoral position
- Students
-
- Matthieu Gratia, PhD. student
- Martin D, Ouldali M, Ménétrey J, Poncet D. Structural organisation of the rotavirus nonstructural protein NSP5. J Mol Biol. 2011. 413: 209-21.
- Rearranged genomic RNA segments offer a new approach to the reverse genetics of rotaviruses. Troupin C, Dehée A, Schnuriger A, Vende P, Poncet D, Garbarg-Chenon A. J Virol. 2010. 84: 6711-9
- Zakhour M., Ruvoen-Clouet N, Charpilienne A, Langpap B, Poncet D, Peters T, Bovin N, LePendu J. The alpha Gal epitope of the histo-blood group antigen family is a ligand for bovine norovirus Newburry2 expected to prevent cross-species transmission. PlosPathogen 2009. 5(7):e1000504
- Harb M, Becker MM, Vitour D, Baron CH, Vende P, Brown SC, Bolte S, Arold ST, Poncet D. Nuclear localization of cytoplasmic poly(A)-binding protein upon rotavirus infection involves the interaction of NSP3 with eIF4G and RoXaN. J Virol. 2008. 82: 11283-93.
- Vitour D, Lindenbaum P, Vende P, Becker MM, Poncet D. RoXaN, a novel cellular protein containing TPR, LD, and zinc finger motifs, forms a ternary complex with eukaryotic initiation factor 4G and rotavirus NSP3. J Virol.. 2004 78: 3851-62
- Vende P, Piron M, Castagne N, Poncet D. Efficient translation of rotavirus mRNA requires simultaneous interaction of NSP3 with the eukaryotic translation initiation factor eIF4G and the mRNA 3' end. J Virol. 2000. 74: 7064-71
- Charpilienne A, Nejmeddine M, Berois M, Parez N, Neumann E, Hewat E, Trugnan G, Cohen J. Individual rotavirus-like particles containing 120 molecules of fluorescent protein are visible in living cells. J Biol Chem. 2001. 276: 29361-7
- Piron M, Vende P, Cohen J, Poncet D. Rotavirus RNA-binding protein NSP3 interacts with eIF4GI and evicts the poly(A) binding protein from eIF4F. EMBO J. 1998. 17: 5811-21
Last modification : - Designed by V. Maury